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Erschienen in:

01.09.2023 | Computed Tomography

Adipose tissue composition determines its computed tomography radiodensity

verfasst von: Amani Zoabi, Einav Bentov-Arava, Adan Sultan, Anna Elia, Ori Shalev, Marina Orevi, Ofer N. Gofrit, Katherine Margulis

Erschienen in: European Radiology | Ausgabe 3/2024

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Abstract

Objectives

Adipose tissue radiodensity in computed tomography (CT) performed before surgeries can predict surgical difficulty. Despite its clinical importance, little is known about what influences radiodensity. This study combines desorption electrospray ionization mass spectrometry imaging (DESI-MSI) and electrospray ionization (ESI) with machine learning to unveil how chemical composition of adipose tissue determines its radiodensity.

Methods

Patients in the study underwent abdominal surgeries. Before surgery, CT radiodensity of fat near operated sites was measured. Fifty-three fat samples were collected and analyzed by DESI-MSI, ESI, and histology, and then sorted by radiodensity, demographic parameters, and adipocyte size. A non-negative matrix factorization (NMF) algorithm was developed to differentiate between high and low radiodensities.

Results

No associations between radiodensity and patient age, gender, weight, height, or fat origin were found. Body mass index showed negative correlation with radiodensity. A substantial difference in chemical composition between adipose tissues of high and low radiodensities was observed. More radiodense tissues exhibited greater abundance of high molecular weight species, such as phospholipids of various types, ceramides, cholesterol esters and diglycerides, and about 70% smaller adipocyte size. Less radiodense tissue showed high abundance of short acyl-tail fatty acids.

Conclusions

This study unveils the connection between abdominal adipose tissue radiodensity and its chemical composition. Because the radiodensity of the fat around the surgical site is associated with surgical difficulty, it is important to understand how adipose tissue composition affects this parameter. We conclude that fat tissue with a higher content of various phospholipids and waxy lipids is more CT radiodense.

Clinical relevance statement

This study establishes the connection between the CT radiodensity of adipose tissue and its chemical composition. Clinicians may use this information for preoperative planning of surgical procedures, potentially modifying their surgical approach (for example, performing partial nephrectomy openly rather than laparoscopically).

Key Points

• Adipose tissue radiodensity values in computed tomography images taken prior to the surgery can potentially predict surgery difficulty.

• Fifty-three human specimens were analyzed by advanced mass spectrometry, molecular imaging, and machine learning to establish the key features that determine Hounsfield units’ values of adipose tissue.

• The findings of this research will enable clinicians to better prepare for surgical procedures and select operative strategies.

Graphical abstract

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Titel: Adipose tissue composition determines its computed tomography radiodensity
verfasst von: Amani Zoabi
Einav Bentov-Arava
Adan Sultan
Anna Elia
Ori Shalev
Marina Orevi
Ofer N. Gofrit
Katherine Margulis
Publikationsdatum: 01.09.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: European Radiology / Ausgabe 3/2024
Print ISSN: 0938-7994
Elektronische ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-023-09911-7

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Adipose tissue composition determines its computed tomography radiodensity