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01.12.2024 | Original Article

An evaluation of photobiomodulation effects on human gingival fibroblast cells under hyperglycemic condition: an in vitro study

verfasst von: Babak Iranpour, Kimia Mohammadi, Mahshid Hodjat, Neda Hakimiha, Ferena Sayar, Mohammad Javad Kharazi Fard, Saeed Sadatmansouri, Reem Hanna

Erschienen in: Lasers in Medical Science | Ausgabe 1/2024

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Abstract

An in vitro study was designed to evaluate the effects of photobiomodulation (PBM) with 915-nm diode laser on human gingival fibroblast (HGF) cells under hyperglycemic condition. The HGF cells were cultured in Dulbecco’s modified eagle medium (DMEM) medium containing 30 mM glucose concentration for 48 h to mimic the hyperglycemic condition. Subsequently, the cells received three sessions of PBM (915 nm, continuous emission mode, 200 mW, energy density values of 3.2, 6, and 9.2 J/cm²). Twenty-four hours post-irradiation, cell proliferation, expression of interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) were assessed with MTT and real-time polymerase chain reaction (PCR) tests, respectively. Also, reactive oxygen species (ROS) production was measured using CM-H2DCFDA fluorimetry. No changes were detected in the cell proliferation rate between the high glucose control group and laser-treated cells, while VEGF and IL-6 gene expression levels increased significantly after PBM in the high glucose-treated cells group. ROS level was significantly decreased in the irradiated cells in high-glucose medium compared with the high glucose control group. Our study revealed the inductive role of 915-nm–mediated PBM on VEGF and the inflammatory response while concurrently reducing reactive oxygen species production in HGF cells in hyperglycemic conditions.

Dhir S, Lalwani R, Sharma JK, Kolte A, Bansal S, Gupta A (2019) “The Perio-Diabetes Symposium”: consensus report of the Indian Society of Periodontology and Research Society for the Study of Diabetes in India-A joint event on Periodontitis and Diabetes. J Indian Soc Periodontol 23:593–594CrossRefPubMedPubMedCentral

Polak D, Sanui T (2000) Nishimura F and Shapira L (2020) Diabetes as a risk factor for periodontal disease—plausible mechanisms. Periodonto 83:46–58CrossRef

Graves DT (2000) Ding Z and Yang Y (2020) The impact of diabetes on periodontal diseases. Periodontol 82:214–224CrossRef

Choi E-J, Yim J-Y, Koo K-T, Seol Y-J, Lee Y-M, Ku Y, Rhyu I-C, Chung C-P, Kim T-I (2010) Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts. J Periodontal Implant Sci 40:105–110CrossRefPubMedPubMedCentral

Basso FG, Pansani TN, Turrioni APS, Bagnato VS, Hebling J, de Souza Costa CA (2012) In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts. Int J Dent 2012:719452. https://doi.org/10.1155/2012/719452CrossRefPubMedPubMedCentral

Li S, Liu X, Zhou Y, Acharya A, Savkovic V, Xu C, Wu N, Deng Y, Hu X, Li H (2018) Shared genetic and epigenetic mechanisms between chronic periodontitis and oral squamous cell carcinoma. Oral Oncol 86:216–224CrossRefPubMed

Artese L, Piattelli A, de Gouveia Cardoso LA, Ferrari DS, Onuma T, Piccirilli M, Faveri M, Perrotti V, Simon M, Shibli JA (2010) Immunoexpression of angiogenesis, nitric oxide synthase, and proliferation markers in gingival samples of patients with aggressive and chronic periodontitis. J Periodontol 81:718–726CrossRefPubMed

Ng Y-S, Krilleke D, Shima DT (2006) VEGF function in vascular pathogenesis. Exp Cell Res 312:527–537CrossRefPubMed

Prapulla DV, Sujatha PB, Pradeep A (2007) Gingival crevicular fluid VEGF levels in periodontal health and disease. J Periodontol 78:1783–1787CrossRefPubMed

10.

Sakallıoğlu EE, Aliyev E, Lütfioğlu M, Yavuz Ü, Açıkgöz G (2007) Vascular endothelial growth factor (VEGF) levels of gingiva and gingival crevicular fluid in diabetic and systemically healthy periodontitis patients. Clin Oral Investig 11:115–120CrossRefPubMed

11.

Naruishi K, Nagata T (2018) Biological effects of interleukin-6 on gingival fibroblasts: cytokine regulation in periodontitis. J Cell Physiol 233:6393–6400CrossRefPubMed

12.

Sawada S, Chosa N, Ishisaki A, Naruishi K (2013) Enhancement of gingival inflammation induced by synergism of IL-1β and IL-6. Biomed Res 34:31–40CrossRefPubMed

13.

Vieira Ribeiro F, de Mendonça AC, Santos VR, Bastos MF, Figueiredo LC, Duarte PM (2011) Cytokines and bone-related factors in systemically healthy patients with chronic periodontitis and patients with type 2 diabetes and chronic periodontitis. J Periodontol 82:1187–1196CrossRef

14.

Dahiya P, Kamal R, Gupta R, Bhardwaj R, Chaudhary K, Kaur S (2013) Reactive oxygen species in periodontitis. J Indian Soc Periodontol 17:411–416CrossRefPubMedPubMedCentral

15.

Liu C, Mo L, Niu Y, Li X, Zhou X, Xu X (2017) The role of reactive oxygen species and autophagy in periodontitis and their potential linkage. Front Physiol 8:439CrossRefPubMedPubMedCentral

16.

Theodoro LH, Marcantonio RAC, Wainwright M, Garcia VG (2021) LASER in periodontal treatment: is it an effective treatment or science fiction? Braz Oral Res 35:e099. https://doi.org/10.1590/1807-3107bor-2021.vol35.0099CrossRefPubMed

17.

Hakimiha N, Dehghan MM, Manaheji H, Zaringhalam J, Farzad-Mohajeri S, Fekrazad R, Moslemi N (2020) Recovery of inferior alveolar nerve by photobiomodulation therapy using two laser wavelengths: a behavioral and immunological study in rat. J Photochem Photobiol B Biol 204:111785CrossRef

18.

Firoozi P, Amiri MA, Soghli N, Farshidfar N, Hakimiha N, Fekrazad R (2022) The role of photobiomodulation on dental-derived mesenchymal stem cells in regenerative dentistry: a comprehensive systematic review. Curr Stem Cell Res Ther. https://doi.org/10.2174/1574888X17666220810141411. Online ahead of print

19.

Pawelczyk-Madalińska M, Benedicenti S, Sălăgean T, Bordea IR, Hanna R (2021) Impact of adjunctive diode laser application to non-surgical periodontal therapy on clinical, microbiological and immunological outcomes in management of chronic periodontitis: a systematic review of human randomized controlled clinical trials. J Inflamm Res 14:2515CrossRefPubMedPubMedCentral

20.

Dalvi S, Benedicenti S, Hanna R (2021) Effectiveness of photobiomodulation as an adjunct to nonsurgical periodontal therapy in the management of periodontitis-a systematic review of in vivo human studies. Photochem Photobiol 97:223–242CrossRefPubMed

21.

Dompe C, Moncrieff L, Matys J, Grzech-Leśniak K, Kocherova I, Bryja A, Bruska M, Dominiak M, Mozdziak P, Skiba THI (2020) Photobiomodulation—underlying mechanism and clinical applications. J Clin Med 9:1724CrossRefPubMedPubMedCentral

22.

Lee KD, Chiang MH, Chen PH, Ho ML, Lee HZ, Lee HE, Wang YH (2019) The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts. Lasers Med Sci 34:913–920. https://doi.org/10.1007/s10103-018-2675-6CrossRefPubMed

23.

Chen H, Sun S, Pan Z, Tu M, Shi J, Bai H, Han Z, Yao J (2021) Effects of photobiomodulation on high glucose induced oxidative stress in human embryonic skin fibroblasts. IEEE J Sel Top Quantum Electron 27:1–9

24.

Mostafavinia A, Ahmadi H, Amini A, Roudafshani Z, Hamblin MR, Chien S, Bayat M (2021) The effect of photobiomodulation therapy on antioxidants and oxidative stress profiles of adipose derived mesenchymal stem cells in diabetic rats. Spectrochim Acta A Mol Biomol Spectrosc 262:120157. https://doi.org/10.1016/j.saa.2021.120157CrossRefPubMed

25.

Esmaeelinejad M, Bayat M (2013) Effect of low-level laser therapy on the release of interleukin-6 and basic fibroblast growth factor from cultured human skin fibroblasts in normal and high glucose mediums. J Cosmet Laser Ther 15:310–317CrossRefPubMed

26.

Houreld NN, Sekhejane PR, Abrahamse H (2010) Irradiation at 830 nm stimulates nitric oxide production and inhibits pro-inflammatory cytokines in diabetic wounded fibroblast cells. Lasers Surg Med 42:494–502CrossRefPubMed

27.

Nobakht-Haghighi N, Rahimifard M, Baeeri M, Rezvanfar MA, Moini Nodeh S, Haghi-Aminjan H, Hamurtekin E, Abdollahi M (2018) Regulation of aging and oxidative stress pathways in aged pancreatic islets using alpha-lipoic acid. Mol Cell Biochem 449:267–276CrossRefPubMed

28.

Sadatmansouri S, Agahikesheh B, Karimi M, Etemadi A, Saberi S (2022) Effect of different energy densities of 915 nm low power laser on the biological behavior of human gingival fibroblast cells in vitro. Photochem Photobiol 98:969–973CrossRefPubMed

29.

Tschon M, Incerti-Parenti S, Cepollaro S, Checchi L, Fini M (2015) Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model. J Biomed Opt 20:078002–078002CrossRef

30.

Boström EA, Kindstedt E, Sulniute R, Palmqvist P, Majster M, Holm CK, Zwicker S, Clark R, Önell S, Johansson I (2015) Increased eotaxin and MCP-1 levels in serum from individuals with periodontitis and in human gingival fibroblasts exposed to pro-inflammatory cytokines. PLoS ONE 10:e0134608CrossRefPubMedPubMedCentral

31.

Esmaeelinejad M, Bayat M, Darbandi H, Bayat M, Mosaffa N (2014) The effects of low-level laser irradiation on cellular viability and proliferation of human skin fibroblasts cultured in high glucose mediums. Lasers Med Sci 29:121–129. https://doi.org/10.1007/s10103-013-1289-2CrossRefPubMed

32.

Silva DFT, Mesquita-Ferrari RA, Fernandes KPS, Raele MP, Wetter NU, Deana AM (2012) Effective transmission of light for media culture, plates and tubes. Photochem Photobiol 88:1211–1216CrossRefPubMed

33.

Koobotse MO, Schmidt D, Holly JM, Perks CM (2020) Glucose concentration in cell culture medium influences the BRCA1-mediated regulation of the lipogenic action of IGF-I in breast cancer cells. Int J Mol Sci 21:8674CrossRefPubMedPubMedCentral

34.

Lewinska A, Wnuk M, Slota E, Bartosz G (2007) Total anti-oxidant capacity of cell culture media. Clin Exp Pharmacol Physiol 34:781–786CrossRefPubMed

35.

Chen H, Tu M, Shi J, Wang Y, Hou Z, Wang J (2021) Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums. Lasers Med Sci 36:555–562. https://doi.org/10.1007/s10103-020-03057-4CrossRefPubMed

36.

Xuan YH, Huang BB, Tian HS, Chi LS, Duan YM, Wang X, Zhu ZX, Cai WH, Zhu YT, Wei TM, Ye HB, Cong WT, Jin LT (2014) High-glucose inhibits human fibroblast cell migration in wound healing via repression of bFGF-regulating JNK phosphorylation. PLoS ONE 9:e108182. https://doi.org/10.1371/journal.pone.0108182CrossRefPubMedPubMedCentral

37.

Wirostko B, Wong TY, Simó R (2008) Vascular endothelial growth factor and diabetic complications. Prog Retin Eye Res 27:608–621. https://doi.org/10.1016/j.preteyeres.2008.09.002CrossRefPubMed

38.

Tsai C-H, Chiang Y-C, Chen H-T, Huang P-H, Hsu H-C, Tang C-H (2013) High glucose induces vascular endothelial growth factor production in human synovial fibroblasts through reactive oxygen species generation. Biochim Biophys Acta 1830:2649–2658. https://doi.org/10.1016/j.bbagen.2012.12.017CrossRefPubMed

39.

Omori K, Naruishi K, Nishimura F, Yamada-Naruishi H, Takashiba S (2004) High glucose enhances interleukin-6-induced vascular endothelial growth factor 165 expression via activation of gp130-mediated p44/42 MAPK-CCAAT/enhancer binding protein signaling in gingival fibroblasts. J Biol Chem 279:6643–6649CrossRefPubMed

40.

Chiu HC, Fu MM, Yang TS, Fu E, Chiang CY, Tu HP, Chin YT, Lin FG, Shih KC (2017) Effect of high glucose, porphyromonas gingivalis lipopolysaccharide and advanced glycation end-products on production of interleukin-6/-8 by gingival fibroblasts. J Periodont Res 52:268–276. https://doi.org/10.1111/jre.12391CrossRef

41.

Lew JH, Naruishi K, Kajiura Y, Nishikawa Y, Ikuta T, Kido J, Nagata T (2018) High glucose-mediated cytokine regulation in gingival fibroblasts and THP-1 macrophage: a possible mechanism of severe periodontitis with diabetes. Cell Physiol Biochem 50:973–986. https://doi.org/10.1159/000494481CrossRefPubMed

42.

Góralczyk K, Szymańska J, Szot K, Fisz J, Rość D (2016) Low-level laser irradiation effect on endothelial cells under conditions of hyperglycemia. Lasers Med Sci 31:825–831. https://doi.org/10.1007/s10103-016-1880-4CrossRefPubMedPubMedCentral

43.

Nibali L, Donos N (2013) Periodontitis and redox status: a review. Curr Pharm Des 19:2687–2697. https://doi.org/10.2174/1381612811319150003CrossRefPubMed

44.

George S, Hamblin MR, Abrahamse H (2018) Effect of red light and near infrared laser on the generation of reactive oxygen species in primary dermal fibroblasts. J Photochem Photobiol B 188:60–68. https://doi.org/10.1016/j.jphotobiol.2018.09.004CrossRefPubMedPubMedCentral

45.

Amaroli A, Ravera S, Baldini F, Benedicenti S, Panfoli I, Vergani L (2019) Photobiomodulation with 808-nm diode laser light promotes wound healing of human endothelial cells through increased reactive oxygen species production stimulating mitochondrial oxidative phosphorylation. Lasers Med Sci 34:495–504. https://doi.org/10.1007/s10103-018-2623-5CrossRefPubMed

Titel: An evaluation of photobiomodulation effects on human gingival fibroblast cells under hyperglycemic condition: an in vitro study
verfasst von: Babak Iranpour
Kimia Mohammadi
Mahshid Hodjat
Neda Hakimiha
Ferena Sayar
Mohammad Javad Kharazi Fard
Saeed Sadatmansouri
Reem Hanna
Publikationsdatum: 01.12.2024
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2024
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-023-03954-4

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An evaluation of photobiomodulation effects on human gingival fibroblast cells under hyperglycemic condition: an in vitro study

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

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