Background
Kawasaki disease (KD) is an acute vasculitis primarily affecting preschool children and predominantly involving small to medium-sized blood vessels. Despite extensive research, the etiology of KD remains unclear [
1]. The primary complication associated with KD is the development ofcoronary artery lesions (CALs), with reported occurrence rates ranging from 15 to 25%. In severe cases, CALs may lead to the rupture of coronary artery aneurysms, myocardial infarction, and even sudden death. Remarkably, KD has emerged as the leading cause of acquired heart disease among children [
2].
The occurrence rate of CALs can be significantly reduced, from 20 to 25% to approximately 2–4%, by combining high-dose intravenous immunoglobulin (IVIG) therapy with aspirin. This treatment strategy has been currently recognized as the first-line approach [
3]. However, a subset of children fails to respond to IVIG, exhibiting persistent fever for more than 36 h after standard treatment or experiencing recurring fever (2–7 days after initial resolution) exceeding 38 °C. This condition, known as IVIG-resistant KD, represents an independent risk factor for CAL development [
4]. Administering corticosteroids early on in these patients has been shown to effectively reduce complications and significantly improve prognosis [
5,
6]. Therefore, accurate prediction of IVIG-resistant KD at an early stage holds great clinical significance, as it allows for intensified early treatment and improved patient outcomes.
As KD is a vasculitis, blood-based markers can provide some insight into its severity. C-reactive protein (CRP) and albumin (ALB) have been included in various risk scoring systems aimed at predicting IVIG resistance in KD patients due to their presumed association with this phenomenon [
7‐
9]. However, previous studies have demonstrated that using CRP or ALB as single indicators for predicting IVIG resistance is not ideal [
10,
11].
Recently, a newly introduced inflammatory parameter called the C-reactive protein-to-albumin ratio (CAR) has shown promise as a more valuable and accurate predictor compared to using CRP or ALB individually. CAR has demonstrated potential in predicting the inflammatory status and prognosis of several clinical diseases [
12‐
14]. Although some studies have investigated the role of CAR in predicting IVIG resistance among KD patients, there is considerable variability in sensitivity (SEN) and specificity (SPE) across these studies, and no systematic review has summarized this evidence to date. Consequently, this study was aimed to collect relevant research results, analyze the data, and conduct a comprehensive meta-analysis to assess the predictive value of CAR in IVIG-resistant KD.
Materials and methods
Protocol
The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Statement was used as a guideline for this study.
Search strategies
We conducted searches in multiple databases, including the Cochrane Library, PubMed, MEDLINE, EMbase, CNKI, WanFang, the Chinese Biomedical Database, and CQVIP, up until November 2023. The search terms used were combinations of the following keywords: children, pediatric; kawasaki disease; C-reactive protein (CRP); albumin (ALB); and C-reactive protein to albumin ratio (CAR). Both subject and free words were used in the search, and the strategies were finalized after conducting several pre-searches. Additionally, we reviewed the references of the included articles and other relevant studies. There were no language restrictions applied.
Study selection
According to the Cochrane Collaboration Network’s systematic evaluation manual, the inclusion and exclusion criteria for this meta-analysis were formulated in strict accordance with the participants, interventions, comparators, outcomes, and study design (PICOS) principles.The inclusion criteria were as follows: (1) The research objectives involved conducting a cohort study to evaluate the early diagnostic value of CAR in IVIG-resistant KD; (2) The study subjects were KD patients who met the relevant diagnostic criteria; (3) Four-fold table data could be directly obtained from the literature or calculated; (4) The sources of reagents and detection methods were clearly defined, and the detected specimens were serum.
The exclusion criteria were as follows: (1) Descriptive studies and case-control studies; (2) Data that was not completely provided or was difficult to extract; (3) Repeated reports (inclusion or exclusion depending on the quality of the literature), reviews, conference papers, or only abstracts where the full text was not available.
Data extraction and quality assessment
For each study, two authors (Jue Liu & Xingguang Chen) independently extracted data and assessed quality. Any disagreements were resolved by the corresponding author (Yiqun Teng & Jian Jin). A standardized data extraction form was used, including:1) Baseline characteristics of the literature: publication date, country or region, number of cases, age, study type, time span, diagnostic criteria, the cut-off value of CAR; (2) Information on diagnostic test parameters: True positive (TP), false positive (FP), false negative (FN), and true negative (TN).
The included studies underwent quality assessment using the Quality Assessment of Diagnostic Accuracy Studies 2.0 (QUADAS-2.0) [
15]. Each item in the included studies was evaluated as “yes”, “no”, or “unclear”. A score of 1 was assigned for “yes,” and 0 for “no” or “unclear.” The literature was considered of higher quality if its score exceeded 7.
Statistical analysis
Stata 15.0 software (StataCorp LLC, North Carolina, USA) was used for statistical analysis. The heterogeneity of the included studies was assessed using the Q test and I2 index. If no heterogeneity (p > 0.1 or I2 < 50% for Q test) was detected for SEN, SPE, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), a fixed-effects model based on the Mantel-Haenszel method was used for pooling. Additionally, the summary receiver operator characteristic (SROC) curve was plotted, and the area under the curve (AUC) was calculated. Otherwise, if there was heterogeneity, the random-effects model using the DerSimonian-Laird method was applied. Clinical utility assessment was conducted using Fagan’s nomogram. Publication bias was assessed using Deek’s funnel plot. Bivariate boxplots were employed to identify literature with high heterogeneity. Cohen’s Kappa analysis was used for the diagnostic consistency analysis.
Discussion
Recently, several studies have investigated the predictive ability of serum inflammatory markers, such as the platelet-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, CRP, ALB, and CAR, in determining the resistance to IVIG treatment in patients with KD [
20‐
22]. Chaudhary H et al. [
23] and Kaya Akca U et al. [
24] conducted comprehensive reviews on potential biomarkers for KD, comparing ten different systems for predicting IVIG resistance. The results of their studies indicated that the predictive ability of these indicators in determining whether KD patients would develop IVIG resistance is unsatisfactory. Although Liu C et al. [
25] performed a meta-analysis on the literature regarding the indicators of PLR, NLR, and their combination for IVIG-resistant KD, they did not analyze the role of CAR.
Our recent studies [
19,
26] have found that CAR demonstrates good predictive capabilities for IVIG resistance and CAL. However, it lacks robust evidence from evidence-based medicine. Therefore, we conducted this meta-analysis with the aim of exploring the ability of CAR to predict IVIG resistance in patients with KD and providing evidence for CAR as an independent predictor or its inclusion in new predictive scoring models, so as to enhance early therapeutic efficacy for KD patients.
This meta-analysis included four studies on CAR predicting IVIG resistance in patients with KD. The pooled results indicated that CAR demonstrated a certain ability to predict IVIG resistance in KD patients (SEN: 0.62, SPE: 0.71). However, at present, CAR can only serve as an auxiliary predictive indicator for the occurrence of IVIG resistance and cannot be used as a definitive diagnostic or exclusionary marker in KD (PLR: 2.22, NLR: 0.49). Overall, CAR has a moderate predictive value for the occurrence of IVIG resistance in KD patients. It was further confirmed by the results of Fagan’s plot.
Therefore, currently, we cannot determine whether CAR can serve as the preferred independent predictive biomarker for predicting IVIG resistance in patients with KD. Establishing a new clinical prediction model that integrates CAR, NLR, and PLR may be an important approach for early prediction of IVIG resistance in KD patients [
27]. This is also the current research direction of our research team.
Combining the results of the QUADAS-2.0 quality assessment, we analyzd possible sources of heterogeneity: (1) Though our study used a random-effects model to minimize the impact of threshold effects, different cut-off values will directly influence the research outcomes; (2) CRP and ALB showed differences in various aspects, such as age, gender, and disease duration [
28]. The ratio between them might amplify or diminish these differences. Expanding the sample size and determining cut-off values based on age or gender are also directions for future CAR research; (3) Search strategies, publication bias, and gray literature might be significant sources of heterogeneity. We have made efforts to obtain gray literature through various means, such as Wang et al. [
18], to minimize heterogeneity. Reducing heterogeneity might have a potential in enhancing the credibility and accuracy of new meta-analyses.
In addition to the high heterogeneity, there were other limitations in this review: (1) All included studies were conducted on Chinese individuals, which might affect the generalizability of the conclusions; (2) There has been a lack of original literature on CAR, requiring further research to draw more definitive conclusions. However, despite these limitations, this meta-analysis alsohad certain strengths. It was the first study to explore the predictive efficiency of CAR in KD patients with IVIG resistance through meta-analysis. The results of our meta-analysis supported CAR as a significant indicator for predicting IVIG resistance in KD patients, thus warranting further in-depth investigation.
Conclusion
In conclusion, our study may contribute to further improving the clinical management of IVIG-resistant KD. The research findings indicated that CAR might bean additional predictive indicator with moderate diagnostic valueto guide early treatment of the disease, demonstrating a certain predictive value for further investigation. However, CAR cannot be considered a definitive diagnostic or exclusionary marker for IVIG-resistant KD at this time. Therefore, multi-center, large sample, and high-quality long-term follow-up trials are warranted to confirm the current findings.
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