Compressed-sensing accelerated 4D flow MRI of cerebrospinal fluid dynamics

Changes in the cerebrospinal fluid (CSF) flow dynamics have been found to be associated with different neurological diseases such as hydrocephalus [1], Chiari malformation [2, 3], syringomyelia [4] and Alzheimer disease [5]. The quantification of the CSF flow is necessary for a better understanding of the physiology and pathophysiology of CSF dynamics and may assist in diagnosis and treatment guidance of CSF related diseases. At present, two-dimensional phase-contrast magnetic resonance imaging (2D flow MRI) is the most common technique to quantify the CSF flow non-invasively [6]. Since this method is two-dimensional it is not sufficient to fully represent the complex physiological or pathological flow dynamics of the CSF including multidirectional flow occurring in patients with Chiari malformation, inside of arachnoid cysts and syringes as well as during reflux from the third into the lateral ventricles [7, 8]. To represent complex flow patterns adequately a time-resolved three-dimensional MRI measurement (4D flow MRI) method is necessary [9, 10]. However, due to the large amount of data that needs to be recorded, the acquisition of a 4D flow MRI data set is accompanied with long scan times and therefore, rarely integrated into clinical routine scan protocols.

To accelerate 4D flow MRI acquisitions, different methods were proposed by exploiting spatial–temporal correlations or using compressed sensing. However, most work focused on cardio-vascular applications [11‐15] and no work exists on accelerated 4D flow MRI of the CSF.

The concept of compressed sensing is a promising way to accelerate MRI measurements. It was first proposed in 2006 by Donoho [16] and transferred to MRI by Lustig et al. [17]. Compressed sensing can reduce the required amount of data by random undersampling of the k-space, if an image can be assumed to be “sparse” in a suitable transform domain [18]. Further image acceleration can be accomplished by combining compressed sensing and parallel imaging [18, 19], herein referred to as compressed SENSE (CSE) [20, 21]. Previous studies have shown that CSE can accelerate scan times while obtaining an acceptable quality in aortic [13, 22, 23] and carotid [24] blood flow using acceleration factors between R = 3 and R = 7. In comparison to blood flow, velocities of the CSF are low and therefore more difficult to measure. Contrast of the tissue is lower and no contrast agent can be used making an exact acquisition more challenging. In addition, when measuring the blood flow in the chest or abdomen more coils can be used which improves quality.

The aim of this study was to investigate the applicability of the combination of compressed sensing and parallel imaging to the quantification of CSF flow using 4D flow MRI to improve its clinical applicability. To estimate the maximum feasible image acceleration using CSE, different CSE factors were compared to the standard parallel imaging acceleration using SENSE regarding image quality and different flow parameters, such as peak velocity, forward volume flow, backward volume flow and absolute net flow in healthy subjects. Finally, to further investigate the potential of the CSE acceleration the proposed method was applied to measure the CSF flow surrounding the entire spinal cord (C1-L1) in one healthy subject by the combination of 2 separate acquisitions.

Scans were completed in all subjects, none of the scans had to be aborted or repeated. In one subject all slices of the second scan using SENSE and in another subject slices from C3–C7 of the scans using SENSE and CSE6 were excluded due to motion artifacts. Acquisition times averaged over all subjects were 10:21± 1:19 min (SENSE), 9:31 ± 1:12 min (CSE4), 6:25 ± 0:51 min (CSE6), 4:53 ± 0:38 min (CSE8) and 3:51 ± 0:29 min (CSE10). The average reconstruction time of CSE accelerated acquisitions took around 10 min per scan.

Figure 2a exemplary shows phase-contrast, magnitude and pathline images of one subject. Figure 2b shows resulting magnitude and phase-contrast images with the corresponding ROI. A decrease in quality with increasing CSE acceleration factor can be noticed. Time resolved animations of the pathlines are available in the supplementary material (see Additional files 1, 2, 3, 4, 5).

Figure 3 shows the time-accumulated flow error averaged over all subjects. While CSE4, CSE6 and CSE8 show comparable flow errors around 30%, the tendency of an increasing error with increasing acceleration can still be observed. The largest deviation can be seen in CSE10 with a flow error of 53.9 ± 39.1%.

Results of the Bland–Altman analyses are shown in Table 2. For Bland–Altman plots see Fig. 4 and Additional files 6, 7, 8.

Limits of agreement (LoA) of CSE4, CSE6 and CSE8 were similar for AF, FF and BF with a slight increase with increasing acceleration factors. In CSE10 a notable widening of the confidence intervals can be observed for all parameters. The PV showed relatively larger confidence intervals than other parameters in all acceleration factors with LoAs between 10–12 cm/s, although the mean differences of PV showed only minor deviations in all acceleration factor (CSE4: 0.3 cm/s; CSE6: 0.43 cm/s; CSE8: 0.33 cm/s; CSE10: − 0.223 cm/s). Confidence intervals (CI) of PV included zero in all acceleration factors. The mean differences in AF and BF were smallest in CSE4 (AF: 0.3 ml/s and BF: 0.3 ml/s), increased in CSE6 (AF:0.8 ml/s; BF: 0.6 ml/s) and were largest in CSE8 (1.1 ml/s and BF: 0.9 ml/s).

Table 3 shows the resulting mean pair differences.

There were no significant differences for PV for all acceleration factors. CSE4, 6 and 8 showed a significant difference for FF of 0.78 ml/s (CSE4), 0.96 ml/s (CSE6) and 0.90 ml/s (CSE8). BF had no significant differences except for CSE8 with an estimated difference of 0.87 ml/s. There was a significant difference for AF for CSE6, 8 and 10, with CSE8 showing the highest difference of 1.13 ml/s (p-value 5.59∙10^–7). The significant difference for CSE6 and 10 was 0.82 ml/s with CIs of 0.37–1.27 ml/s (CSE6) and 0.15–1.49 ml/s (CSE10). There is a tendency to underestimate values in all CSE acceleration factors.

The test–retest comparison (see Fig. 4b) showed small differences between the first and the second scan using SENSE in all parameters (mean differences: AF: 0.08 ml/s; PV: − 0.32 ml/s; FF: − 0.003 ml/s; BF: − 0.215 ml/s). CIs included 0 in all parameters. In all parameters the LoA for repeated scans using SENSE were comparable to the LoA in CSE8. The standard deviation (SD) of repeated scans using SENSE acquisitions was higher than in scans using CSE4, CSE6 and CSE8. Mean pair difference showed no significant differences between the first and the second scan using SENSE in all parameters assessed.

Data covering the C1-L1 region were acquired successfully. The acquisition time took 7:20 min per stack and 14:40 min in total. Time-resolved animations of recorded pathlines are available in the supplementary material (see Additional file 9). A screenshot of the pathlines is shown in Fig. 5.

To our knowledge, this is the first study evaluating the feasibility of CSE acceleration for 4D flow MRI of CSF flow dynamics. Moreover, by applying an appropriate CSE acceleration, we were able to demonstrate that it is feasible to measure the CSF flow surrounding the entire spinal cord during a single examination with a clinically acceptable scan time.

Peak flow velocities (5.6 ± 2.7 cm/s) were in good agreement with results of previous studies by Pahlavian et al. [27] with a peak velocity of 6.0 ± 1.7 cm/s, by Bunck et al. [9] with values between 3.0 and 4.8 cm/s and by Lindstrøm et al. [28] with values between 1.8 and 4.2 cm/s.

The flow parameters analyzed in this study showed a good test–retest agreement between repeated scans using SENSE. The SD of 3.16 cm/s for PV and around 2 ml/s for BF, AF and FF seem high compared to the CSE data. This can be attributed to both the smaller number of subjects in the test–retest group naturally leading to wider CIs and the physiological fluctuations due to the delay between the acquisitions of the first and the second scan using SENSE. To minimize physiological differences, like respiration or varying heart rate, the order of acquisitions was randomized except for the second SENSE accelerated scan. This may explain an increased SD of the second scan using SENSE compared to the scans using CSE4 and CSE6. Additionally, reduced movement and physiological variations due to the shorter scan time in scans using CSE4 and CSE6 may lead to increased accuracy. This could also serve as a possible explanation why CSE10 showed no significant differences for FF, BF and PV.

Overall Bland–Altman analyses showed good agreement between scans using CSE and SENSE with acceleration factors up to CSE8. The LoA and the SD of the PV are relatively wider than for the rest of the parameters. This finding can most likely be attributed to the internal process of determining this specific value. The PV is defined as the pixel with the highest velocity. Therefore, this parameter can be subject to large fluctuations due to noise. Another reason that could contribute to deviations is the temporal resolution and the temporal smoothing by 4D flow PCMRI [29, 30]. This finding was also observed in the ventricular structures by Stadlbauer et al. [10] and in the cerebral aqueduct by Sartoretti et al. [31]. Although in all parameters the mean difference of CSE10 is comparable to CSE4 and CSE6 and showed no significant difference for FF, BF, and PV, wide confidence intervals, a high accumulated flow error and a substantially decreased image quality led to the conclusion that CSE10 is beyond adequate acceleration factors. SD of the scan-rescan measurement was between 1.77 and 2.29 ml/s. There were no significant differences for CSE4 for BF and AF and for CSE6 for BF. The significant differences that were detected for CSE4 and CSE6 are small (MD < 0.5 ml/s for CSE4, MD < 0.9 ml/s for CSE6). Those deviations are probably clinically not relevant as the alterations in flow velocities such as in stenotic flow are usually well beyond these minor deviations [2]. Small CIs indicate a higher accuracy due to lower variations within different subjects.

Results of the accumulated flow error were broadly in line with results of the Bland–Altman analyses. A flow error around 30% is higher than reported for cardiovascular 4D flow MRI, for which errors between 15 and 20% in aortic flow and an error between 20–30% in the pulmonary arteries have been described [11, 26]. This can most likely be attributed to the CSF flow being more sensitive to disturbances and the smaller flow velocities making stronger gradients necessary [8]. Additionally, CSF velocities are close to zero in a large portion of the cardiac cycle, which increases the measurement error when using a PCMRI sequence with single encoding velocity. Also, outlining of the spinal CSF can be challenging, e.g. by the contamination of adjacent blood vessels [9], especially because we chose to shift the contours manually and not automatically. Previous studies have shown that respiration also effects the CSF flow in both direction and speed [32‐34]. This might be an additional cause for larger errors between repeated scans. Although CSE6 and CSE8 showed similar results in the Bland–Altman analysis and the accumulated flow error, defining contours in images acquired with CSE8 may be difficult due to the reduced magnitude image quality (see Fig. 2). This could potentially lead to greater deviations than observed in our study, as all contours were outlined in the SENSE accelerated scan and also needs to be considered when choosing the appropriate acceleration factor. Moreover, in contrast to CSE8, mean deviations were less than 10% of absolute values for all tested parameters when applying CSE6 acceleration. Additionally, CSE8 showed a significant difference for AF, FF and BF with a mean difference > 1 ml/s for AF whereas CSE6 only showed a significant difference for AF and FF with mean differences always < 1 ml/s. Compared to SENSE and CSE4, the CSE6 scan achieved a noticeable reduction in scan time. It is possible that the reduced scan time is the reason for the lower accumulated flow error and may improve image quality by reduced motion artifacts. It would also be possible to make use of the time gained in CSE6 by e.g. choosing a higher temporal resolution. Accordingly, we regard CSE6 as the most appropriate acceleration factor.

Although only the cervical spine was assessed in detail in our study, we have demonstrated the feasibility of a measurement covering the entire spinal cord and its surrounding CSF space in two stacks in 14 min. Such an acquisition allows for a more comprehensive visualization of CSF dynamics and may provide important diagnostic information for patients with syringomyelia, Chiari malformation or arachnoid adhesions [35‐37].

Our study has several limitations. The study assessed only a relatively small number of healthy subjects. However, the results of our study clearly show the feasibility of CSE6 accelerated 4D flow MRI of the CSF dynamics at the cervical spinal canal. With a higher number of subjects, we would expect standard deviations and level of agreements to be even smaller. Since no patient data were recorded, these results are not fully transferable to patients with abnormal, more complex flow dynamics. With CSE6, however, we have chosen a rather conservative acceleration factor, so that its use in everyday clinical practice seems conceivable. Quantitative analysis was only provided for the CSF flow at the cervical spinal canal. However, as the feasibility of an imaging volume covering the entire spinal cord was shown in one subject with sufficient image quality, the results are likely to be applicable to whole spine measurements. A specific challenge of whole spine measurements is the correct cardiac gating of both stacks. This could reduce the accuracy of the correct flow dynamics. A major limitation is the missing respiratory gating. Studies by Spijkerman et al. [38] and Yildiz et al. [39] have shown that respiratory gated 2D flow MRI measurements provide more accurate quantifications of peak velocity, net flow, and stroke volume in 2D acquisitions with significant but small differences between respiratory gated acquisitions in inspiration and expiration. However, respiratory gating prolongs the scan time by about a factor of 2 to 3 and thus leads to extremely increased scan times in 4D flow MRI. Therefore, non-respiratory gated 4D flow MRI acquisitions were acquired to achieve clinical feasible scan times. Future studies may enable further acceleration, making respiratory gated 4D flow MRI acquisitions of the spinal CSF feasible with scan times of less than 10 min and thus enable more accurate quantifications of CSF hydrodynamics.

We have chosen a relatively high parallel imaging factor (SENSE) in order to achieve an acquisition with a similar duration as CSE4. A longer SENSE scan with lower acceleration factors would have exceeded the duration of the entire scan protocol. Additionally, although the image quality is increased by lower acceleration factors, the influence of motion artifacts may be more prevalent with increased scan time.

Finally, further sequence optimizations are still applicable. Long reconstruction times can be further improved by application of dedicated reconstruction algorithms [40] and temporal-spatial redundancy may be exploited leading to increased image acceleration [41].

Our study demonstrates that CSE is a reliable method to accelerate CSF flow measurements. Acceleration factors up to CSE6 seem to be appropriate for clinical use enabling a reduction of acquisition time by 40%. Scan times of about 6 min for the acquisition of a cervical spine imaging volume and about 14 min for a set of two imaging volumes covering the entire spinal cord can be achieved. This facilitates the use of 4D flow MRI for the diagnosis of pathologic CSF dynamics as in Chiari Malformation or syringomyelia.