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European Archives of Oto-Rhino-Laryngology

Erschienen in:

31.05.2023 | Miscellaneous

Degenerative changes induced by paradoxical sleep deprivation in rat sublingual glands

verfasst von: Gabriel Carvalhal de Aguiar, Ana Carolina Flygare Souza, Daniel Vitor de Souza, Marcos Monico Neto, Luciana Le Sueur-Maluf, Andrea Cristina de Moraes Malinverni, Hanna Karen Moreira Antunes, Daniel Araki Ribeiro

Erschienen in: European Archives of Oto-Rhino-Laryngology | Ausgabe 9/2023

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Abstract

Purpose

The aim of this study was to evaluate whether sleep deprivation can induce degenerative changes in rat sublingual glands.

Methods

For this purpose, a total of 24 males were distributed into three groups: control (n = 8), in which the animals were not subjected to any procedure; sleep deprivation (n = 8) in which the animals were submitted to sleep deprivation for 96 h; recovery (n = 8), in which the animals were subjected to paradoxical sleep deprivation for 96 consecutive hours followed by 96 h without intervention. Morphological changes in sublingual glands as well as the immunoexpressions of some proteins, such as Ki-67, p16, cleaved caspase-3 and BCL-2 were investigated in this setting.

Results

The results showed that paradoxical sleep deprivation induced tissue degeneration as a result of the presence of pyknosis, vacuoles and areas of salivary retention, in the experimental groups. Expression of cleaved caspase 3 and BCL-2 were increased in both sleep deprivation and recovery groups. The analysis of Ki-67 showed an increase in expression only in the recovery group, associated with a decrease in p16 levels.

Conclusion

Sleep deprivation can induce a degenerative process in the parenchyma of sublingual gland by means of dysregulation of apoptosis associated with proliferative activity.

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Titel: Degenerative changes induced by paradoxical sleep deprivation in rat sublingual glands
verfasst von: Gabriel Carvalhal de Aguiar
Ana Carolina Flygare Souza
Daniel Vitor de Souza
Marcos Monico Neto
Luciana Le Sueur-Maluf
Andrea Cristina de Moraes Malinverni
Hanna Karen Moreira Antunes
Daniel Araki Ribeiro
Publikationsdatum: 31.05.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: European Archives of Oto-Rhino-Laryngology / Ausgabe 9/2023
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-023-08041-6

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Degenerative changes induced by paradoxical sleep deprivation in rat sublingual glands