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08.04.2024 | Original Article

Effect of initial periodontal therapy on metallothionein levels in smokers and non-smokers with periodontitis

verfasst von: Vikender Singh Yadav, Anu Bhatia, Rakhee Yadav, Kanika Makker, Dhiraj Kumar Singh, Riyaz Ahmad Mir

Erschienen in: Odontology

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Abstract

The aim of this study was to determine the effect of non-surgical periodontal therapy (NSPT) on mRNA expression of metallothionein (MT) and its levels in serum, saliva and gingival crevicular fluid (GCF) of smokers (S) and non-smokers (NS) with periodontitis (P).

A total of 100 participants were included: 48 periodontally healthy (PH) subjects (24 S [PH + S] and 24 NS [PH + NS]) and 52 patients with P (27 S [P + S] and 25 NS [P + NS]). Clinical parameters were recorded, and biofluids (serum, saliva and GCF) and gingival tissue samples were obtained at baseline in all groups and 3 months after NSPT in P groups. MT levels in biofluids were determined by ELISA. In gingival tissues, MT-mRNA expression was quantified using real-time PCR. mRNA expression of MT and its levels in biofluids were significantly higher in P + S compared to other groups, and the differences between P + NS and PH + S were non-significant. A significant decrease was observed for MT levels in biofluids, and MT-mRNA expression in periodontitis patients after NSPT. In conclusion, smoking and periodontitis are associated with higher MT expression which decreases after NSPT. MT as an oxidative stress biomarker and its therapeutic role in periodontitis should be investigated in future studies.

Clinical trial registration: The study was prospectively registered at Clinical Trials Registry—India (ctri.nic.in) as CTRI/2018/08/015427 on August 23, 2018.

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Titel: Effect of initial periodontal therapy on metallothionein levels in smokers and non-smokers with periodontitis
verfasst von: Vikender Singh Yadav
Anu Bhatia
Rakhee Yadav
Kanika Makker
Dhiraj Kumar Singh
Riyaz Ahmad Mir
Publikationsdatum: 08.04.2024
Verlag: Springer Nature Singapore
Erschienen in: Odontology
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-024-00937-x

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Effect of initial periodontal therapy on metallothionein levels in smokers and non-smokers with periodontitis

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Abstract

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Darf man die Behandlung eines Neonazis ablehnen?

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

Endlich: Zi zeigt, mit welchen PVS Praxen zufrieden sind

Parodontalbehandlung verbessert Prognose bei Katheterablation

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