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07.06.2022 | Original Article

Effects of different photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study

verfasst von: Mylene Martins Monteiro, Juliana Amorim dos Santos, Victor Paiva Barbosa, Camila de Barros Gallo, Márcia Martins Marques, Eliete Neves Silva Guerra

Erschienen in: Lasers in Dental Science | Ausgabe 3/2022

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Abstract

Purpose

Photobiomodulation therapy has proven to be effective in accelerating cell proliferation, migration, and transcription. The study aimed to analyze the cell viability effects of different parameters of PBMT in a cultured cell line of human gingival fibroblasts after bacterial and ionizing radiation–induced stress.

Methods

Explant technique was used to produce a primary cell culture. Cells were grown in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum until stressful condition induction with lipopolysaccharide of Escherichia coli, Porphyromonas gingivalis protein extract, and ionizing radiation. Laser irradiation was carried out in four sessions set with 660 nm wavelength, an output power of 30 mW and 40 mW and energy density of 2, 3, 4, and 5 J/cm².

Results

After 24 h from the last laser irradiation session, the groups outputted in 30 mW of power maintained the cell viability while operating with 2, 4, and 5 J/cm². However, 3 J/cm² dose significantly decreased cell viability (p < 0.05). When the laser irradiation session was set in a higher power (40 mW), cell viability was reduced using 2, 3, and 5 J/cm² doses, with statistical significance for 5 J/cm² (p < 0.001). In addition, operating the same energy using lower power seems to be superior to a higher power, being statically significant for 5 J/cm² dose (p < 0.001). This pattern followed with all different groups, except by 3 J/cm².

Conclusions

The present study showed that delivering 2, 4, and 5 J/ cm² of density of energy with 30 mW and more time of exposure presented better results on cell viability compared to the same density of energy with output power of 40 mW. Further studies comparing density energy should be conducted.

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Titel: Effects of different photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study
verfasst von: Mylene Martins Monteiro
Juliana Amorim dos Santos
Victor Paiva Barbosa
Camila de Barros Gallo
Márcia Martins Marques
Eliete Neves Silva Guerra
Publikationsdatum: 07.06.2022
Verlag: Springer International Publishing
Erschienen in: Lasers in Dental Science / Ausgabe 3/2022
Elektronische ISSN: 2367-2587
DOI: https://doi.org/10.1007/s41547-022-00162-1

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Effects of different photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study