No relationship between early postnatal testosterone concentrations and autistic traits in 18 to 30-month-old children

One of the most consistent findings in autism spectrum conditions (ASCs) research is that males are more likely than females to be diagnosed with the conditions [1]. In addition, in the general population, males on average score higher on measures of autistic traits than females [2‐5].

Studying the causal mechanisms involved in this gender bias may provide insights into the aetiology of ASCs. It has been proposed that ASCs reflect an ‘extreme male brain (EMB)’ and that androgens during early development are a key neurobiological factor underlying development of an EMB [6, 7]. Some prior studies have found that testosterone prenatally is significantly related to subsequent autistic traits [8‐11]. A recent study compared 128 males with an ASC diagnosis to 217 matched controls without an ASC diagnosis and found no significant difference in testosterone that had been measured in their amniotic fluid [12]. That same study, however, found that the ASC group scored significantly higher than the controls on a latent steroidogenic factor that was derived from measures of five hormones (progesterone, 17α-hydroxy-progesterone, androstenedione, testosterone, and cortisol) assayed in amniotic fluid [12].

Little is known about whether androgen exposure during early infancy relates to later autistic traits. There is a transient surge of sex steroids, called ‘mini-puberty’ , in male infants, with testosterone concentrations peaking at about 1 to 3 months postnatal, before declining to low childhood values by around 6 months of age [13, 14]. Testosterone during mini-puberty has been shown to influence development of the male genitalia and reproductive function [15] and has been associated with postnatal penile and testicular growth in healthy infants [16]. Since brain plasticity remains high throughout the early postnatal period [17], it is possible that mini-puberty contributes to neurobehavioral sexual differentiation, as well.

Two studies have examined the influences of testosterone during mini-puberty on autistic traits. These studies related testosterone assayed from saliva samples collected at 3 to 4 months of age to parent-reported autistic traits during toddlerhood. The first study found no correlation between testosterone and scores on the Quantitative Checklist for Autism in Toddlers (Q-CHAT) in boys (n = 15), girls (n = 20), or the entire sample at age 18 to 35 months [8]. The second study reported a significant positive correlation between testosterone and scores on a subscale of the Brief Infant–Toddler Social and Emotional Assessment (BITSEA) in 47 boys and 37 girls combined at age 18 to 24 months but did not report any within-sex correlation [18]. Notably, neither study found a sex difference in testosterone concentrations, perhaps because saliva samples were collected after the peak of mini-puberty. One of the studies also found no gender difference in autistic traits [18]. The lack of differences between boys and girls limits the studies’ implications for the neurobehavioral effects of mini-puberty on autistic traits.

The present study explored the relationship between salivary testosterone during the peak of mini-puberty, at 1 to 3 months of age, and parent-reported autistic traits, as assessed by the Q-CHAT, at 18 to 30 months of age. Saliva sampling was employed since it affords a non-invasive approach. The Q-CHAT was used because prior studies have found gender differences in Q-CHAT scores [2, 8].

There were no outliers and all variables had acceptable skewness. Boys and girls differed significantly in testosterone concentrations and in Q-CHAT scores but not in any other variable (see Table 1 for descriptive, independent sample t test and Cohen’s d statistics). There were no significant bivariate correlations between Q-CHAT scores and testosterone or any control variable in boys, girls, or the entire sample (see Table 2 for Pearson’s correlation statistics and Fig. 1 for a scatter plot showing the relationship between Q-CHAT scores and testosterone within each sex).

Since the Q-CHAT was originally designed for children at the age of 18 to 24 months [2], the above analyses were repeated excluding children older than 24 months (12 boys, 12 girls). Highly comparable results were produced. There were no significant correlations between testosterone and Q-CHAT scores in boys (r = .09), girls (r = .03), or the entire sample (r = .13) for children ages 18 to 24 months.

The present study investigated whether salivary testosterone measured at 1 to 3 months of age relates to autistic traits assessed by the Q-CHAT at 18 to 30 months of age. Consistent with prior studies [2, 8], the present study found that boys scored higher on the Q-CHAT than girls. Whereas prior studies reported no sex difference in salivary testosterone in infants at age 3 to 4 months [8, 18], the present study found that boys had higher concentrations of salivary testosterone than girls at age 1 to 3 months, during the peak of the early postnatal surge (mini-puberty). Despite detecting differences between boys and girls in both testosterone and autistic traits, the present study found no correlation between testosterone and autistic traits in boys, girls, or the entire sample. Although the current results cannot be directly compared with prior findings, due to the differences in the timing of testosterone measures, research findings thus far appear to suggest that testosterone during mini-puberty has little to no effects on autistic traits [8, 18].

In addition to augmenting evidence on testosterone and autistic traits, our findings highlight the potential of saliva sampling as an approach for studying the effects of early androgen exposure on neurobehavioural sexual differentiation. The present study detected a sex difference in salivary testosterone, which resembles prior findings showing sex differences in testosterone in blood [13, 14] and urine [21] samples collected during the peak of the postnatal surge. Because saliva can be obtained non-invasively and conveniently, its use may be particularly well-suited for further research investigating the effects of mini-puberty. Whilst mini-puberty may not contribute to autistic traits, it has been found that urinary testosterone and penile growth during mini-puberty predict gender-typed play behaviour in early childhood [21, 22]. Further research may relate salivary testosterone to other aspects of development that differ by gender.

The current results do not address the influences of testosterone prenatally. There is some evidence suggesting that testosterone exposure during foetal development may relate to later autistic traits [8‐12]. By contrast, prior studies found no relationship between autistic traits and perinatal or postnatal androgen exposure, as measured in umbilical cord blood at birth or saliva after the peak of mini-puberty [8, 18, 23, 24]. Moreover, the present study found no relationship between testosterone at the peak of mini-puberty and autistic traits. Thus, research evidence thus far appears to suggest that early postnatal testosterone does not contribute to autistic traits.

Limitations of the present study include reliance on a small sample, which could reduce statistical power, and the largely Caucasian and highly educated participants (see Table 1), which could reduce generalisability of the findings. Further research with larger and more diverse samples would be of interest.

In conclusion, the present study expands on prior research, suggesting that there is no relationship between testosterone exposure during mini-puberty and autistic traits. This does not preclude effects of mini-puberty on other behaviours, however. Indeed, testosterone during mini-puberty has been found to predict gender-typed play behaviour. Future studies might use early postnatal saliva samples to evaluate the effects of mini-puberty on other traits that show gender differences.