Erschienen in:
01.12.2024 | Original Article
Photobiomodulation effects of blue light on osteogenesis are induced by reactive oxygen species
verfasst von:
Maria Albaqami, Blanche Aguida, Ayda Pourmostafa, Margaret Ahmad, Vipuil Kishore
Erschienen in:
Lasers in Medical Science
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Ausgabe 1/2024
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Abstract
Blue light–mediated photobiomodulation (PBM) is a promising approach to promote osteogenesis. However, the underlying mechanisms of PBM in osteogenesis are poorly understood. In this study, a human osteosarcoma cell line (i.e., Saos-2 cells) was subjected to intermittent blue light exposure (2500 µM/m2/s, 70 mW/cm2, 4.2 J/cm2, once every 48 h) and the effects on Saos-2 cell viability, metabolic activity, differentiation, and mineralization were investigated. In addition, this study addressed a possible role of blue light induced cellular oxidative stress as a mechanism for enhanced osteoblast differentiation and mineralization. Results showed that Saos-2 cell viability and metabolic activity were maintained upon blue light exposure compared to unilluminated controls, indicating no negative effects. To the contrary, blue light exposure significantly increased (p < 0.05) alkaline phosphatase activity and Saos-2 cell mediated mineralization. High-performance liquid chromatography (HPLC) assay was used for measurement of reactive oxygen species (ROS) activity and showed a significant increase (p < 0.05) in superoxide (O2•−) and hydrogen peroxide (H2O2) formed after blue light exposure. Together, these results suggest that the beneficial effects of blue light–mediated PBM on osteogenesis may be induced by controlled release of ROS.