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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Erschienen in:

11.02.2022 | Original Article

Testosterone suppression impacts craniofacial growth structures during puberty

An animal study

verfasst von: Caio Luiz Bitencourt Reis, Magali de Fátima Pereira Madureira, Caio Luis Rocha Cunha, Wagner Costa Rossi Junior, Tomaz Henrique Araújo, Alessandra Esteves, Maria Bernadete Sasso Stuani, Christian Kirschneck, Peter Proff, Mírian Aiko Nakane Matsumoto, Erika Calvano Küchler, Daniela Silva Barroso de Oliveira, DDS, MS, PhD

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Ausgabe 5/2023

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Abstract

Aim

Hormones play a crucial role in growth development; however, the impact of testosterone suppression (TS) on craniofacial growth during puberty remains inconclusive. This study aimed to evaluate the impact of TS during puberty on cephalometric measurements and histological characteristics of facial growth centers.

Materials and methods

Thirty-six heterogenic Wistar male rats were randomly allocated into experimental orchiectomy (ORX) and control (sham) groups. At an age of 23 days (prepubertal stage), orchiectomy and placebo surgery were performed. Cephalometric measurements were performed via lateral cephalograms during and after puberty. The animals were euthanized at an age of 45 days (pubertal stage) and 73 days (postpubertal stage). Histological slices of the growth centers (condyle, premaxilla, and median palatine suture) were stained with hematoxylin and eosin, and sirius red. Student’s t or Mann–Whitney U tests were used to compare linear and angular cephalometric measurements across groups (α error = 5%).

Results

Linear and angular measurements were statistically different in ORX animals (cranial bones, maxilla, and mandible) at 45 days and 73 days. Condylar histology showed a decrease in prechondroblast differentiation and a delay of mineralization in ORX animals. Vascularization of the medium palatine suture was lower in the ORX group at 45 days. Type I and III collagen fiber synthesis was lower in the ORX groups. In the premaxillary suture, collagen fibers were better organized in the sham groups.

Conclusions

Our results suggest that testosterone suppression affects craniofacial growth during puberty.

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Titel: Testosterone suppression impacts craniofacial growth structures during puberty
An animal study
verfasst von: Caio Luiz Bitencourt Reis
Magali de Fátima Pereira Madureira
Caio Luis Rocha Cunha
Wagner Costa Rossi Junior
Tomaz Henrique Araújo
Alessandra Esteves
Maria Bernadete Sasso Stuani
Christian Kirschneck
Peter Proff
Mírian Aiko Nakane Matsumoto
Erika Calvano Küchler
Daniela Silva Barroso de Oliveira, DDS, MS, PhD
Publikationsdatum: 11.02.2022
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe 5/2023
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-021-00373-4

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Springer Medizin

Testosterone suppression impacts craniofacial growth structures during puberty

An animal study

Abstract