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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Erschienen in:

22.03.2022 | Original Article

Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

verfasst von: Lili Li, Peihong Liu, Xuechao Lv, Tianliang Yu, Xingai Jin, Rui Wang, Xiaohua Xie, Qingshan Wang, Yingqun Liu, Wuliji Saiyin

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Ausgabe 6/2023

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Abstract

Short root defects are prone to cause various periodontal diseases and lead to tooth loss in some serious cases. Studies about the mechanisms governing the development of the root are needed for a better understanding of the pathogenesis of short root defects. The protein family with sequence similarity 20 group C (FAM20C) is a Golgi casein kinase that has been well studied in the development of tooth crown formation. However, whether FAM20C plays a role in the development of tooth root is still unknown. Thus, we generated Sox2-Cre;Fam20c^fl/fl (cKO) mice, in which Fam20c was ablated in both the dental epithelium and dental mesenchyme, and found that the cKO mice showed severe short root defects mainly by inhibiting the development of dental mesenchyme in the root region. In this investigation, we found morphological changes and differentiation defects, with reduced expression of dentin sialophosphoprotein (DSPP) in odontoblasts of the root region in cKO mice. Furthermore, the proliferation rate of apical papillary cells was reduced in the root of cKO mice. In addition, the levels of bone morphogenetic protein 4 (BMP4) and phospho-Smad1/5/8, and that of Osterix and Krüppel-like factor 4 (KLF4), two downstream target molecules of the BMP signaling pathway, were significantly reduced in the root of cKO mice. These results indicate that FAM20C plays an essential role in the development of the root by regulating the BMP signaling pathway.

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Titel: Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice
verfasst von: Lili Li
Peihong Liu
Xuechao Lv
Tianliang Yu
Xingai Jin
Rui Wang
Xiaohua Xie
Qingshan Wang
Yingqun Liu
Wuliji Saiyin
Publikationsdatum: 22.03.2022
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe 6/2023
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-022-00386-7

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Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

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